Permeabilization ofCochliomyia hominivorax(Diptera: Calliphoridae) Embryos

Permeabilization of Cochliomyia hominivorax (Diptera: Calliphoridae) embryos.

Embryos of the primary screwworm, Cochliomyia hominivorax (Coquerel), were successfully permeabilized for use in subsequent cryopreservation studies. Mortality was greater for eggs incubated for < 5 h before treatment. The mean survival of embryos to first instars was 55.7, 61.1, and 62.6% when the embryos were incubated for 5, 5.5, and 6 h before treatment, respectively. The survival to the pu...

Cryopreservation of embryos of Lucilia sericata (Diptera: Calliphoridae).

Embryos of Lucilia (Phaenicia) sericata (Meigen) (Diptera Calliphoridae), the green blowfly, were successfully cryopreserved by vitrification in liquid nitrogen and stored for 8 yr. Embryos incubated at 19 degrees C for 17 h after oviposition were found to be the most appropriate stage to cryopreserve. Removal of the embryonic surface water was done using 2-propanol before the alkane treatment ...

Truncation of screwworm (Diptera: Calliphoridae) development by irradiation of embryos.

PHYSIOLOGY, BIOCHEMISTRY, AND TOXICOLOGY Cryopreservation of Embryos of the New World Screwworm Cochliomyia hominivorax (Diptera: Calliphoridae)

A method for cryopreserving embryos of the screwworm, Cochliomyia hominivorax (Coquerel), was developed for the long-term storage of strains used in research projects and for colonies maintained as back-up to production strains that are mass-reared for sterile insect release programs. This protocol, when combined with a previous procedure that was designed to render screwworm embryos permeable ...

Cryopreservation of Embryos of the New World Screwworm <i>Cochliomyia hominivorax</i> (Diptera: Calliphoridae)

A method for cryopreserving embryos of the screwworm, Cochliomyia hominivorax (Coquerel), was developed for the long-term storage of strains used in research projects and for colonies maintained as back-up to production strains that are mass-reared for sterile insect release programs. This protocol, when combined with a previous procedure that was designed to render screwworm embryos permeable ...